Born Tomaso D'Antonio Manzuoli in 1536, the artist was a key figure in the Florentine school during the mid-16th century. He received his training in Florence, where he developed a style characterized by the refined, elongated forms and sophisticated color palettes typical of the Mannerist movement.

His work is primarily associated with religious commissions and portraiture, often reflecting the aesthetic preferences of the Florentine court. He was notably involved in the collaborative projects for the Studiolo of Francesco I de' Medici in the Palazzo Vecchio, which remains one of his most recognized contributions to the period's artistic legacy.